{"id":692,"date":"2023-03-23T13:43:40","date_gmt":"2023-03-23T13:43:40","guid":{"rendered":"https:\/\/students.pingry.org\/irt\/?page_id=692"},"modified":"2023-03-31T12:58:07","modified_gmt":"2023-03-31T12:58:07","slug":"fluorescent-fish-2022-3-project","status":"publish","type":"page","link":"https:\/\/students.pingry.org\/irt\/fluorescent-fish-2022-3-project\/","title":{"rendered":"Fluorescent Fish – 2022\/3 Project"},"content":{"rendered":"

[et_pb_section fb_built=”1″ admin_label=”Hero” _builder_version=”4.4.7″ background_color=”#f8fbfe” use_background_color_gradient=”on” background_color_gradient_start=”#53a0fd” background_color_gradient_end=”#002960″ background_color_gradient_direction=”74deg” background_image=”https:\/\/students.pingry.org\/irt\/wp-content\/uploads\/sites\/19\/2020\/07\/geometric-bg-overlay-01.jpg” background_blend=”overlay” custom_padding=”2.4%|0px||0px||” animation_style=”zoom” animation_direction=”bottom” animation_duration=”0ms” animation_intensity_zoom=”0%” animation_starting_opacity=”100%” box_shadow_style=”preset7″ box_shadow_horizontal=”0px” box_shadow_vertical=”-200px” box_shadow_color=”#F8FBFE”][et_pb_row _builder_version=”4.4.7″ custom_padding=”||0px|||”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.4.7″ text_font=”Abhaya Libre||||||||” text_text_color=”#ffffff” text_font_size=”18px” text_line_height=”1.8em” header_font=”Nunito Sans|700|||||||” header_text_align=”center” header_text_color=”#ffffff” header_font_size=”45px” header_line_height=”1.3em” header_2_font=”||||||||” text_orientation=”center” max_width=”700px” module_alignment=”center” custom_margin=”||18px|||” custom_padding=”||17px|||” inline_fonts=”Abhaya Libre”]<\/p>\n

Fluorescent Fish<\/span>\u00a02022-2023 Project – Expression and Analysis of\u00a0Fluorescent Fish Proteins in Vitro<\/span><\/span><\/h1>\n

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Rachel Zhang (Form VI), Morgan McDonald (Form VI), Annika Shekdar (Form V), Ethan Boroditsky (Form V)<\/span><\/h3>\n

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Project Summary + Project Goal\u00a0<\/h1>\n

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Fluorescent Fish is made possible by the discovery of the fluorescent eel in 2013, the first fluorescent marine animal. Since then, the lizardfish was discovered, which is another fluorescent organism, whose protein we are focusing on. We are hoping to express novel fluorescent proteins from fluorescent fish and learn about their fluorescent properties. <\/span><\/h2>\n

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Current State<\/h2>\n

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\u00a0This past year, we were able to get expression and purification of the protein. We also concluded that the protein was soluble, which is a great indicator that we can move forward with our research, and potentially insert a fluorescent cofactor into the protein. <\/span><\/h2>\n

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Next Steps<\/span><\/h2>\n

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We will express the fluorescent fatty-acid binding protein on a larger scale and then design a protocol for inducing fluorescence by adding biliverdin. Several other similar proteins will also be expressed and analyzed. <\/span><\/h2>\n

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Research Week Poster\u00a0<\/span><\/h2>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.4.7″ custom_margin=”|auto|-38px|auto||”][et_pb_column type=”4_4″ _builder_version=”4.4.7″][et_pb_image src=”https:\/\/students.pingry.org\/irt\/wp-content\/uploads\/sites\/19\/2023\/03\/Fluorescent-Fish-2022-research-week-poster-Rachel-Zhang.jpg” title_text=”Fluorescent Fish 2022 research week poster – Rachel Zhang” _builder_version=”4.4.7″ custom_margin=”-39px|||||” border_width_all=”4px” border_style_all=”inset”][\/et_pb_image][et_pb_text _builder_version=”4.4.7″ header_font=”Abhaya Libre|600|||||||” header_2_font=”Abhaya Libre|600|||||||” header_2_font_size=”54px” inline_fonts=”Abhaya Libre”]<\/p>\n

References<\/span><\/h2>\n

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Baker, Monya. \u201cFirst Fluorescent Protein Identified in a Vertebrate.\u201d Nature, 13 June 2013, www.nature.com\/articles\/nature.2013.13190, 10.1038\/nature.2013.13190. Accessed 6 Apr. 2022. <\/span><\/a><\/span><\/h2>\n

Gruber, David F., et al. \u201cAdaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.\u201d PLOS ONE, vol. 10, no. 11, 11 Nov. 2015, p. e0140972, journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0140972, 10.1371\/journal.pone.0140972. Accessed 7 Apr. 2022. <\/span><\/a><\/span><\/h2>\n

Remington, S. James. \u201cGreen Fluorescent Protein: A Perspective.\u201d Protein Science, vol. 20, no. 9, 19 July 2011, pp. 1509\u20131519, www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3190146\/, 10.1002\/pro.684. Accessed 7 Apr. 2022. <\/span><\/a><\/span><\/h2>\n

Rodriguez, Erik A., et al. \u201cThe Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.\u201d Trends in Biochemical Sciences, vol. 42, no. 2, Feb. 2017, pp. 111\u2013129, www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5272834\/, 10.1016\/j.tibs.2016.09.010. Accessed 6 Apr. 2022. <\/span><\/a><\/span><\/h2>\n

Ye, Songtao, et al. \u201cPrinciples, Modulation, and Applications of Fluorescent Protein Chromophores.\u201d Chemical Physics Reviews, vol. 3, no. 1, Mar. 2022, p. 011308, aip.scitation.org\/doi\/10.1063\/5.0080417, 10.1063\/5.0080417. Accessed 7 Apr. 2022.<\/span><\/a><\/span><\/h2>\n

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Meet the Team<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.4.7″ column_structure=”1_4,1_4,1_4,1_4″][et_pb_column _builder_version=”4.4.7″ type=”1_4″][et_pb_image src=”https:\/\/students.pingry.org\/irt\/wp-content\/uploads\/sites\/19\/2023\/03\/G11-Zhang-R.jpg” _builder_version=”4.4.7″ title_text=”G11 Zhang R” hover_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column _builder_version=”4.4.7″ type=”1_4″][et_pb_image src=”https:\/\/students.pingry.org\/irt\/wp-content\/uploads\/sites\/19\/2023\/03\/G11-McDonald-M.jpg” _builder_version=”4.4.7″ title_text=”G11 McDonald M” hover_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column _builder_version=”4.4.7″ type=”1_4″][et_pb_image src=”https:\/\/students.pingry.org\/irt\/wp-content\/uploads\/sites\/19\/2023\/03\/G10-Shekdar-A.jpg” _builder_version=”4.4.7″ title_text=”G10 Shekdar A” hover_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column _builder_version=”4.4.7″ type=”1_4″][et_pb_image src=”https:\/\/students.pingry.org\/irt\/wp-content\/uploads\/sites\/19\/2023\/03\/G10-Boroditsky-E.jpg” _builder_version=”4.4.7″ title_text=”G10 Boroditsky E” hover_enabled=”0″][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_6,1_6,1_6,1_6,1_6,1_6″ _builder_version=”4.4.7″ min_height=”23px” custom_margin=”|auto|-416px|auto||” custom_padding=”||16px|||”][et_pb_column type=”1_6″ _builder_version=”4.4.7″ custom_padding=”||0px||false|false”][et_pb_text _builder_version=”4.4.7″ header_2_font=”Abhaya Libre|600|||||||” header_2_text_color=”#ffffff” header_2_font_size=”35px” width=”120%” custom_margin=”-2px||||false|false” custom_padding=”|49px||0px||”][\/et_pb_text][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.4.7″][et_pb_text _builder_version=”4.4.7″ header_font=”Abhaya Libre|600|||||||” header_2_font=”Abhaya Libre|600|||||||” header_2_text_color=”#ffffff” header_2_font_size=”35px” width=”157.7%” custom_margin=”-43px|8px||-175px|false|false” custom_padding=”|0px||||” hover_enabled=”0″]<\/p>\n

Rachel Zhang\u00a0<\/h2>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.4.7″][et_pb_text _builder_version=”4.4.7″ header_font=”Abhaya Libre|600|||||||” header_2_font=”Abhaya Libre|600|||||||” header_2_text_color=”#ffffff” header_2_font_size=”35px” width=”132.4%” custom_margin=”-44px|8px||-62px|false|false” custom_padding=”|0px||||” hover_enabled=”0″]<\/p>\n

Morgan McDonald\u00a0<\/h2>\n

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Annika Shekdar<\/h2>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_6″ _builder_version=”4.4.7″][et_pb_text _builder_version=”4.4.7″ header_font=”Abhaya Libre|600|||||||” header_2_font=”Abhaya Libre|600|||||||” header_2_text_color=”#ffffff” header_2_font_size=”35px” width=”132.4%” custom_margin=”-40px|8px||-70px|false|false” custom_padding=”|0px||||” hover_enabled=”0″]<\/p>\n

Ethan Boroditsky<\/h2>\n

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Dr. Daniel Fried\u00a0<\/h2>\n

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Fluorescent Fish\u00a02022-2023 Project – Expression and Analysis of\u00a0Fluorescent Fish Proteins in VitroRachel Zhang (Form VI), Morgan McDonald (Form VI), Annika Shekdar (Form V), Ethan Boroditsky (Form V)Project Summary + Project Goal\u00a0Fluorescent Fish is made possible by the discovery of the fluorescent eel in 2013, the first fluorescent marine animal. Since then, the lizardfish was discovered, […]<\/p>\n","protected":false},"author":68,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-692","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/pages\/692","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/users\/68"}],"replies":[{"embeddable":true,"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/comments?post=692"}],"version-history":[{"count":12,"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/pages\/692\/revisions"}],"predecessor-version":[{"id":823,"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/pages\/692\/revisions\/823"}],"wp:attachment":[{"href":"https:\/\/students.pingry.org\/irt\/wp-json\/wp\/v2\/media?parent=692"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}